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. 2023 Sep 18;14:5781. doi: 10.1038/s41467-023-41529-7

Fig. 5. Therapeutic potentials of Pg-Fe-hMSC in both monocellular and multicellular humanized fibrotic models.

Fig. 5

a Schematic illustration and representative image of EpCAM immunostaining in primary human lung epithelial cells (hLECs). Scale bar, 50 μm. b Mitochondrial transfer rates from the indicated hMSC to the primary hLEC (n = 3 biologically independent cells). c Relative intracellular ROS levels (n = 3 biologically independent cells), d TGF-β expression levels (n = 4 biologically independent cells), and e Viability of BLM-treated hLEC after the indicated treatment using different engineered hMSCs (n = 3 biologically independent cells). f Schematic illustration showing the preparation of the 3D multicellular human fibrotic model. g Representative immunostaining images showing the expression of α-smooth muscle actin (α-SMA), vimentin, collagen-I, and Ki-67 in the healthy and fibrotic multicellular human spheroid models. Blue fluorescent signals indicate the cell nuclei and green signals indicate the biomarkers. Scale bar, 20 μm. h Mitochondrial transfer rates of different engineered hMSC in fibrotic human lung spheroids (n = 3 biologically independent experiments). i Relative intracellular ROS levels (n = 3 biologically independent experiments) and j TGF-β expression levels of fibrotic human lung spheroids after the indicated treatment using different engineered hMSCs (n = 4 biologically independent experiments). k Viability of fibrotic human lung spheroids after the indicated treatment using different engineered hMSCs (n = 3 biologically independent experiments). Data are presented as means ± SD. Statistical significance was analyzed using ordinary one-way ANOVA. ECs epithelial cells.