FIG. 1.

Representative immunoblots of individual human serum specimens, obtained in Connecticut during 1995 and 1996, showing variations in total antibody responses to lysates of the NCH-1 strain of the HGE organism. Molecular masses are indicated in kilodaltons. Lanes 2, 4 to 7, and 9 to 11, reactivities of seropositive specimens. Protein antigen with a molecular mass of about 44 kDa was frequently reactive. The sample in lane 8 shows a single weak band at about 100 kDa, but the sample was recorded as negative. The human serum samples in lanes 1, 3, and 12 show no reactivity. Lane 13, a positive control for HGE.