Detection of sulfite formation in the MaSqoD -catalyzed SQ cleavage by a colorimetric Fuchsin assay
(A) UV-vis absorption spectra of the complete assay and the negative controls omitting MaSqoD or SQ are shown in red, blue, and black, respectively.
(B) Absorbance at 580 nm of each assay. Inset: Photographs of reaction mixtures. The complete assay 1, the negative control omitting SQ 2, and the negative control omitting SqoD 3. Data are represented as mean ± SD. See also Figures S3 and S4.