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. 2023 Aug 31;26(10):107803. doi: 10.1016/j.isci.2023.107803

Figure 3.

Figure 3

LC-MS detection of the SqoD reaction product 6-dehydroglucose

(A) HPLC elution profiles of the DNPH derivatized products of the SqoD reaction, monitoring the absorbance at 360 nm, showing product peaks for 1 and 2 in the full assay, corresponding to two stereoisomers of DNPH-6-dehydroglucose.

(B) Extracted ion chromatograms (m/z (−) 356.9, the predicted mass of the DNPH-6-dehydroglucose monoanion), showing the same two product peaks 1 and 2.

(C–E) The ESI m/z (−) spectrum of the region spanned by three peaks in Figure 3B, showing a species with m/z (−) 356.9 corresponding to different isomers of DNPH-6-dehydroglucose (two possible isomers are depicted). See also Figure S5.