Skip to main content
. 2023 Aug 28;30:286–300. doi: 10.1016/j.omto.2023.08.012

Figure 2.

Figure 2

Smoking induces WEE1 protein expression in EAC cells

(A) Western blot analysis of WEE1, p-CDC2 (Y15), and β-actin in FLO-1 and OE33 cells treated with nicotine for 1 or 3 h. (B) Bblot analysis of WEE1, p-CDC2 (Y15), and β-actin in FLO-1 and OE33 cells treated with NNK for different time points. (C–E) Western blot analysis of WEE1, p-CDC2 (Y15), and β-actin in FLO-1, OE33, and CPB cells treated with 2% cigarette smoking extract (CSE). (F) Immunofluorescence staining of WEE1 (green) in FLO-1 cells treated with 2% CSE or NNK. Scale bar, 50 μm. (G) Quantification of (F) ∗p<0.05, ∗∗p<0.01.