Figure 5.

WEE1 inhibitor MK1775 overcomes smoking-induced docetaxel resistance in vitro

(A) Graphic summary of the hypothesis that smoking induces WEE1 expression promoting docetaxel drug resistance in EAC cells. (B and C) ATP-Glo cell viability assay analysis of IC₅₀ values of MK-1775, or docetaxel (DTX) in EAC cells. (D and E) ATP-Glo cell viability assay analysis of docetaxel IC₅₀ values in FLO-1 or OE33 control or CSE long-term (CSE-LT)-treated cells. (F) Western blot results of WEE1, PARP, cleaved PARP, caspase-3, cleaved caspase-3, and β-actin in FLO-1 and OE33 control or CSE-LT cells treated with docetaxel overnight. (G) SynergyFinder analysis showed a significant synergy effect between WEE1 inhibitor MK-1775 and docetaxel in FLO-1 CSE-LT cells. (H) Clonogenic assay in OE33 cells treated with docetaxel or combined with MK-1775. (I) Quantification of (H). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.