Figure 2.

Successful copulation decreases female receptivity

(A) Events of successful copulation over time when mated (red) and virgin (blue) females were paired with males (n = 23).

(B) Elapsed time before reaching successful copulation when mated (red) and virgin (blue) females were paired with males (n = 23).

(C) Courting attempts of males on mated (red) and virgin (blue) females (n = 8–9).

(D) The frequency of pausing per minute of mated (red) and virgin (blue) females toward the pursuing males (n = 8–9).

(E) The frequency of VPO bouts per minute of mated (red) and virgin (blue) females toward the pursuing males (n = 8–9).

(F) The frequency of OE bouts per minute of mated (red) and virgin (blue) females toward the pursuing males (n = 8–9). Wild type males and females were used in (A)-(F). All experimental conditions are indicated with the plots. In the box-and-whisker plot, the whiskers mark the minimum and maximum, the box includes the 25th to 75th percentiles, and the line within the box indicates the median of the data set. The Log-Rank test was applied to (A). The unpaired t-test was performed for (B)-(F). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001.