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. 2023 Sep 4;14:1254677. doi: 10.3389/fimmu.2023.1254677

Figure 2.

Figure 2

Representative images of skin tissue from European sea bass (Dicentrarchus labrax) bath-challenged with 5 x 105 CFU mL-1 T. maritimum. (A) Heavy infiltration of inflammatory cells in the dermis of challenge fish at 24 h. H–E. Bar 50 μm. (B) Extensive necrosis of the dermis associated with infiltration of inflammatory cells in the hypodermis of challenge fish at 24 h. H–E. Bar 50 μm. (C) Immunohistochemistry against T. maritimum antigen, revealing extensive proliferation of T. maritimum in the dermis of challenged fish at 24 h, with agglomerates of bacteria in the epidermis and scale pockets (*) Bar 50 μm. (D) Necrosis and agglomerates of bacteria in the dermis with infiltration of inflammatory cells in the hypodermis. Bar 50 μm. (E) Vacuolization of epithelial cells from the epidermis of challegend fish at 24 h (arrrow), with agglomerates of T. maritimum in the same area. Bar 50 μm. (F) Proliferation of these bacteria in challenged fish at 48 h post-challenge. Bar 20 μm. Section subjected to immunocytochemistry against T. maritimum antigen.