Fig. 2.

mTreg and emTreg bind higher amounts of HDL than nTreg. Purified Treg subsets from healthy controls were incubated with HDL-DiI in X-VIVO medium at 4 or 37°C. Cells were stained with anti-CD4 and NucBlue to visualize membrane (red) and nucleus (purple) and analyzed by Imagestream. A: Representative example of staining of one cell in each subset. B: MFI of HDL-DiI in all three subsets is shown for one representative individual. C: MFI of HDL-DiI binding in each condition (n = 3). Asterisks indicate significant differences (∗∗P < 0.005, ∗∗∗P < 0.0005) in two-way ANOVA.