Fig. 3.

Akt blockage attenuates the HDL antiapoptotic effect on mTreg and emTreg. Purified bulk Treg subsets from healthy controls were pretreated with MK-2206 (Akt inhibitor [AKTi], 3 μM) in X-VIVO medium for 1 h at 37°C. HDL (700 μg/ml of protein) was added, and the cells were incubated overnight. Caspase 3/7 expression of Treg subsets is shown in the figure. Asterisks indicate significant differences (∗P < 0.05, ∗∗P < 0.005, ∗∗∗∗P < 0.0001) in paired t-test (n = 7).