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. 2023 Aug 12;64(9):100425. doi: 10.1016/j.jlr.2023.100425

Fig. 6.

Fig. 6

Total mTreg cells bind high amount of lipid-free rhAPOE3 but not rhAPOA1. Sorted nTreg (CD127−CD25+CD45RA+CD95−) and total mTreg (CD127−CD25+CD45RA−CD95+) were incubated with either rhAPOE3 or rhAPOA1 labeled with Alexa Fluor 488 for 1 h at 37°C in X-VIVO medium. Cells were stained with anti-CD4 (red) and NucBlue (purple) to visualize membrane and nucleus and analyzed by Imagestream. A: Representative example of each subset. B and C: MFI of fluorescent rhAPOE3 bound to either nTreg or mTreg. D: Representative example of one cell for each apolipoprotein. E and F: MFI of rhAPOE3- or rhAPOA1-bound mTreg. Asterisks indicate significant differences (∗P < 0.05) in t-test (n = 3). G: Representative example of one cell for rhAPOE3 or rhAPOE3 + RAP (LRPs inhibitor, 10 µM). H and I: MFI of rhAPOE3 bound to mTreg pretreated with or without RAP. Nonsignificant differences in t-test (n = 3).