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. 2023 Sep 19;23:882. doi: 10.1186/s12885-023-11361-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Immunohistochemistry and q-PCR verified the expression level of YTHDF3 between TC and normal tissues. A Immunohistochemical expression of YTHDF3 on TC (PTC, FTC, ATC, MTC) tissues. The brown area in Fig. 6A marked the positive staining of YTHDF3 in tumor tissues. The scale bar is located in the lower right corner of each group of images, with a scale of 100 μm under 100x microscope and 20 μm under 400x microscope. B The quantification of immunohistochemistry based on positive area score. C The expression levels of YTHDF3 mRNA in human PTC, FTC, ATC and MTC cell and the normal thyroid follicular epithelial cell were analyzed by q-PCR. The data represents the mean ± S.E.M. (* p < 0.05, **p < 0.01, ***p < 0.001). The human PTC cell line K1, human FTC cell line FTC-238, human ATC cell line 8305 C, human MTC cells TT and normal human thyroid follicular epithelial cell line Nthy-ori3-1 were used in q-PCR. (* p < 0.05, **p < 0.01, ***p < 0.001)