Table 1.
HPLC–DAD analysis. Results of M. sylvestris leaf extracts expressed as ng per mg of FMW
| Chemical compound | WL* | 50WEL | 50WEL aq | 70WEL | 70WEL aq | EL | EL aq |
|---|---|---|---|---|---|---|---|
| Gallic acid | 0.36 ± 0.02 | 0.04 ± 0.002 | 10.03 ± 0.05 | 0.3 ± 0.001 | 2.51 ± 0.012 | n.d. | 1.39 ± 0.07 |
| 3-Hydroxytyrosol | 0.042 ± 0.02 | n.d. | 5.5 ± 0.03 | n.d. | 1.84 ± 0.01 | n.d. | n.d. |
| 4-Hydroxybenzoic acid | 1.27 ± 0.06 | 5.36 ± 0.03 | 6.71 ± 0.03 | 1.25 ± 0.006 | 0.53 ± 0.003 | n.d. | 0.52 ± 0.003 |
| Chlorogenic acid | 4.2 ± 0.0.21 | 0.05 ± 0.0002 | 6.57 ± 0.03 | n.d. | 0.16 ± 0.006 | 2.18 ± 0.01 | n.d. |
| Caffeic Acid | 0.1 ± 0.0001 | n.d. | 0.12 ± 0.001 | 11.3 ± 0.06 | 0.91 ± 0.004 | 2.52 ± 0.01 | 0.75 ± 0.004 |
| Vanillic acid | 0.2 ± 0.001 | n.d. | 0.27 ± 0.001 | n.d. | 2.02 ± 0.01 | n.d. | 1.66 ± 0.01 |
| Epicatechin | 39.3 ± 0.19 | n.d. | 4.98 ± 0.02 | 59.2 ± 0.3 | 2.42 ± 0.01 | 13.18 ± 0.06 | n.d. |
| Syringic acid | 0.01 ± 0.00005 | 29.14 ± 0.14 | 0.3 ± 0.001 | 56.37 ± 0.3 | 1.9 ± 0.001 | 0.19 ± 0.01 | 0.15 ± 0.001 |
| p-Coumaric acid | 0.27 ± 0.014 | 3.82 ± 0.2 | 0.28 ± 0.001 | 2.43 ± 0.01 | 0.35 ± 0.002 | 0.24 ± 0.01 | 0.26 ± 0.001 |
| Salicylic acid | 2.96 ± 0.01 | 33.79 ± 0.12 | 2.82 ± 0.01 | 32.8 ± 0.1 | n.d. | 3.5 ± 0.02 | n.d. |
| Quercetin-3-glucoside | 3.91 ± 0.02 | 146.9 ± 0.73 | 2.81 ± 0.01 | 159.8 ± 0.8 | 0.67 ± 0.03 | 27.03 ± 0.13 | 0.83 ± 0.004 |
| Myricetin | 0.53 ± 0.003 | 0.29 ± 0.0014 | 1.41 ± 0.01 | 6.5 ± 0.03 | 14.5 ± 0.07 | 0.62 ± 0.003 | 0.87 ± 0.004 |
| Rosmarinic acid | 0.21 ± 0.001 | 0.11 ± 0.0005 | 0.56 ± 0.003 | 6.6 ± 0.03 | 5.76 ± 0.03 | 0.25 ± 0.001 | 0.34 ± 0.002 |
| Quercetin | 1.9 ± 0.01 | 6.18 ± 0.03 | 4.62 ± 0.02 | 5.1 ± 0.03 | 3.39 ± 0.02 | 0.78 ± 0.004 | 1.68 ± 0.01 |
| 5,7-Dimethoxycoumarin | 5.51 ± 0.03 | 15.32 ± 0.07 | 0.59 ± 0.003 | 16.5 ± 0.08 | 1.14 ± 0.06 | 9.54 ± 0.05 | n.d. |
| Genistein | n.d. | n.d. | 2.59 ± 0.01 | 1.2 ± 0.006 | 2.01 ± 0.01 | n.d. | 0.23 ± 0.001 |
| Kaempferol | n.d. | 0.78 ± 0.004 | 1.25 ± 0.01 | 1.9 ± 0.01 | 2.5 ± 0.01 | 2.15 ± 0.01 | 1.23 ± 0.006 |
| 1,1-Dimethylallyl caffeate | 0.01 ± 0.005 | 0.54 ± 0.0003 | 0.035 ± 0.002 | 0.15 ± 0.001 | 0.28 ± 0.001 | 1.63 ± 0.01 | 0.28 ± 0.001 |
| Chrysin | n.d. | 0.21 ± 0.001 | 0.1 ± 0.0005 | 0.16 ± 0.001 | 0.01 ± 0.005 | 0.22 ± 0.001 | 0.1 ± 0.0005 |
| Caffeic acid phenethyl ester | n.d. | 0.35 ± 0.002 | 0.55 ± 0.003 | 0.29 ± 0.001 | 0.04 ± 0.002 | 3.0 ± 0.01 | 0.37 ± 0.002 |
*Legend—W 100% ddH2O, 50WE 50% ethanol, 70WE 70% ethanol, E 95% ethanol, L leaf, aq aqueous extract, n.d. not detected