Fig. 7.

⁸⁹Zr-oxine radiolabeling did not interfere with the biological functional potency of CAR-T cells. A In vitro potency assay of radiolabeled and unlabeled CAR-T cells showed no significant difference in their IL-13Rα2 positive target tumor cell killing abilities in a co-culture assay as described in Materials and Methods. B Co-culture assay of labeled and unlabeled CAR-T effector cells with IL-13Rα2 positive U251, A172 and U87MG target cells showed similar values of IFN-γ release in 20-h culture. IL-13Rα2 negative T98G glioma cells co-cultured with labeled and unlabeled CAR-T cells secreted basal amounts of IFN-γ. A representative data of three independent experiments performed in quadruplicate is shown