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. 2023 Aug 10;10(9):uhad162. doi: 10.1093/hr/uhad162

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Construction of a cDNA library and screening of transcription factors using Y1H. (a) Screening efficiency of the CsTCS1 promotor library; the dilution factor from top to bottom is 10×, 100×, and 1000×, respectively. (b) Results of self-activation detection of bait colonies, 3-amino-1,2,4-triazole (3-AT) resistant yeast bait strains grown on SD/−Trp-Leu-His (SD-TLH) media supplemented with different concentrations of 3-AT (0, 50, 100 nM). (c) Screening of the constructed cDNA library for transcription factors on SD-TLH media containing 50 nM 3-AT. Negative controls, pGAD53m + pHis2; positive controls, pGAD53m + p53His.