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. 2023 Aug 10;10(9):uhad162. doi: 10.1093/hr/uhad162

Figure 3.

Figure 3

Validation of the transcriptional activity of CsS40. (a) Detection of the interaction between CsS40 and the TCS1 promoter. Yeast cells expressing the pGADT7-BD vector were used as a negative control. (b) Pro-TCS1 was shown to associate with CsS40 using a split-luciferase complementation assay. Nicotiana benthamiana leaves were transformed with Agrobacterium EHA105 cells harboring the Pro-TCS1-nLUC and cLUC-35S:CsS40 plasmids. The combination of Pro-TCS1-nLUC and cLUC-35S: CsS40 resulted in robust luciferase complementation; however, the negative control produced no obvious signal. (c) In a split luciferase complementation assay, N. benthamiana leaves were transformed by injection of Agrobacterium EHA105 cells harboring Pro-TCS1-nLUC and cLUC-35S:CsS40 plasmids. the letters A-d and A-c indicate whether there is significant difference (P < 0.05). (d) Different DNA fragments and cis-acting elements of promoter. (e) EMSA verified the interaction between CsS40 and promoter of TCS1.1: positive probe+ positive protein; P1–P7: binding of different DNA fragments of promoters to S40 protein.