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. 2023 Aug 10;10(9):uhad162. doi: 10.1093/hr/uhad162

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of the overexpression and silencing of CsS40 in tea calli on the expression of TCS1 and the accumulation of caffeine. (a) Calli transformed with the pSH737-35S-CsS40 overexpression plasmid or the pBWA-35S-CsS40 silencing plasmid were cultured on MS medium containing 0.5 mg/L 6-BA, 1.0 mg/L NAA, 200 mg/L Tim, and 30 mg/L Kan. Subsequently, GUS staining of transgenic tea plant calli was performed. (b) RT-qPCR was used to evaluate the expression levels of CsS40 and TCS1 in calli. WT, non-transgenic callus as a control; CspSH, transgenic callus overexpressing CsS40; CspBWA, transgenic callus harboring silenced CsS40; **, extremely significant differences between values. (c) HPLC analysis of caffeine content in callus of CspSH and CspBWA.