FIG. 3.

Detection of minor resistant subpopulation by direct inoculation of a positive blood culture into the Bactometer. The blood isolate (E. cloacae 0333) was resistant to gentamicin (curve B), cefamandole (curve C), and cefotaxime (curve D). However, the DTs in the presence of cefamandole (9 h) and cefotaxime (10.3 h) were much longer than that (3.5 h) for the positive control. The MICs (cefamandole, 128 μg/ml; cefotaxime, 512 μg/ml) determined for subcultures obtained from the module wells were much higher than those (cefamandole, 16 μg/ml; cefotaxime, 1 μg/ml) for organisms subcultured from the original blood bottle. The detection time of positive control (curve A) was 3.5 h.