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. Author manuscript; available in PMC: 2023 Oct 19.
Published in final edited form as: Neuron. 2022 Aug 29;110(20):3374–3388.e8. doi: 10.1016/j.neuron.2022.08.001

Figure 1: LC to dCA1 projecting cells are required for contextual memory linking, but not for contextual conditioning.

Figure 1:

(A) Exploration of a novel context increased cFos expression in the TH+ cells of LC (unpaired t-test, n=5, ***p<0.001). Example images of TH and cFos staining in LC. Scale bar, 50μm. TH- magenta, cFos- cyan. The LC is outlined.

(B) Schematics of experimental design for surgery. Example image for virus spread in HP estimated with AAV-DIO-GFP injected together with CAV-cre. Scale bar, 300μm.

(C) Inhibition of LC cells projecting to dCA1 during exploration of context A impaired contextual memory linking tested with a 5 hours interval. (Control, n=17; LC inhibited, n=15. Two-way repeated measures ANOVA, Sidak post hoc. ##p <0.01, ***p<0.001, ****p<0.0001). Context A- Ctx A, Context B- Ctx B, Context C- Ctx C (neutral). CNO-Clozapine-N-oxide was given to all mice. * is used to depict significance within groups and # is used to show significance between groups for two-way RM ANOVA.

(D) Inhibition of LC cells projecting to dCA1 did not affect contextual conditioning (Two-way RM ANOVA, Sidak posthoc test; control, n=7; LC inhibited, n=7, **p<0.001). CNO-Clozapine-N-oxide was given to all mice.

All results are mean ± s.e.m.