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Real‐time PCR primer sequences and thermocycling condition.
| Genes | Name primer | Primer sequence (5′–3′) | Accession no. | Tem. primer (°C) |
|---|---|---|---|---|
| SOD | F | TGGCGAAGAAGGCTGTTTGT | JF342355AJ | 60.4 |
| R | TTCACTGGACCCGTCT | |||
| CAT | F | CTGGAAGTGGAATCCGTTTG | JF411604 | 54.5 |
| R | CGACCTCAGCGAAATAGTTG | |||
| GPX | F | CCTTCCCATCCCACCAGTTT | FJ656211 | 60 |
| R | TGCGGAGTCACCGTTCACAT | |||
| β‐actin | F | CGTGATGGACTCTGGTGATG | M24113 | 60 |
| R | TCGGCTGTGGTGGTGAAG |
Abbreviations: CAT, catalase; GPX, glutathione peroxidase; SOD, superoxide dismutase.
