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. 2023 Sep 14;150(18):dev201666. doi: 10.1242/dev.201666

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Planarian glial cells develop after neurons during embryogenesis. (A) Single embryo RNA-sequencing shows expression of neuronal and glial markers (re-analysis of data from Davies et al., 2017). (B) In situ hybridization of neuronal (ChAT) and glial (if-1, calamari and estrella) markers in planarian embryos (early S6, mid-S6, S7, S7.5 and S8) and juveniles (1 week post-hatching); ventral views. Arrowheads indicate estrella expression in mouth (S7) and peripheral nervous system (S8). (C) In situ hybridization of ChAT and estrella on staged embryos; dorsal views. Higher magnification images show expression near and within the eyespot. estrella expression near the eye appears at S8 (arrowhead); ChAT expression, in contrast, is seen as early as S7. Anterior is leftwards. Scale bars: 200 µm; 50 µm (higher magnification images).