Figure 1: C. neoformans Glucuronoxylomannan (GXM) Synthetic Decasaccharide (GXM10-Ac₃) resonances are resolved through ¹H-¹³C CT-cHSQC and HSQC TOCSY experiments.

(A) GXM10-Ac₃ chemical structure contains the M2 motif, a common serotype A GXM repeating unit, and binds strongly to anti-GXM Abs (21). Glycans are color coded as follows: Man (green), Xyl (orange), and GlcA (blue). Transglycosidic φ and ψ torsions are denoted on the first three residues. (B-C) Comparison of resolutions achieved by a standard ¹H-¹³C HSQC experiment relative to a CT-cHSQC experiment, with the latter allowing assignments of previously overlapping signals in a few hours for Xyl[II, IV, X]-2 (B) and Xyl[II,IV, VIII]-3 (C). (D) ¹H-¹H crosspeaks between Xyl H1 and H3 obtained from a ¹H-¹³C HSQC TOCSY experiment allows further differntiation between Xyl[II] and Xyl[III] H2C2 resonances.