Figure 2. ParSE-seq assay in HEK cells and iPSC-CMs.

A) Detailed schematic of the minigene plasmid. An 18-mer barcode inserts into downstream rat insulin exon 2.

B) Overview schematic of assembly and assay steps, and subsequent integration. Dashed lines represent amplicons for long- and short-read sequencing.

C) Barcode counts for the assembly, and recovered barcodes present across three replicates in HEK and iPSC-CM assays.

D) Unique WT or variant inserts covered by barcodes in panel C.

E) PSI for all WT exons in iPSC-CMs and HEK cells. Data is averaged across three replicates and error bars represent the standard error of the mean.