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. 2023 Sep 19;14:5808. doi: 10.1038/s41467-023-41591-1

Fig. 2. Cryo-EM characterizations for Fmoc–F–F (His).

Fig. 2

a Cryo-EM image of Fmoc–F–F (His) showing primitive spindle filament with heterogeneous diameter. b Two-dimensional classification analysis of Fmoc–F–F (His) showing parallel alignment and entangled bundles in spindle structure. The images correspond to three morphologies (dashed yellow circle, dashed red circle, and dashed blue circle) of boundles in a, respectively. c Negatively stained Cryo-EM image of Fmoc–F–F (His) using uranium acetate confirming the spindle structure of nanofilaments. Three times each experiment was repeated independently with similar results. Representative images are shown.