TABLE 2.
Antibiotic resistance patterns and hybridization of S. pneumoniae penicillin-resistant isolates recovered in Italy with ermB, mefE, tetM, and cat probes
| Strain code | MIC (mg/liter) ofa:
|
Hybridization with resistance probesb
|
Chromosomal location (PFGE SmaI band) (kb)
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PEN | ERY | CHL | TET | CO-TX | ermB | mefE | cat | tetM | ermB | mefE | cat | tetM | |
| GEN 1R | 2 | 0.12 | 8 | 2 | 16 | − | − | + | + | 388 | |||
| GEN 2R | 2 | 0.12 | 8 | 2 | 16 | − | − | + | + | 388 | |||
| GEN 3R | 2 | 0.12 | 8 | 2 | 8 | − | − | + | + | 388 | |||
| GEN 4R | 2 | 0.12 | 8 | 4 | 16 | − | − | + | + | 388 | |||
| GEN 5Rc | 4 | >128 | 2 | 64 | 8 | + | − | − | + | 122 | |||
| GEN 6Rc | 4 | >128 | 2 | 64 | 8 | + | − | − | + | 122 | |||
| GEN 7Rc | 2 | >128 | 2 | 64 | 8 | + | − | − | + | 122 | |||
| GEN 8Rc | 4 | >128 | 2 | 64 | 8 | + | − | − | + | 122 | |||
| GEN 9Rc | 2 | >128 | 2 | 64 | 8 | + | − | − | + | 122 | |||
| GEN 10Rc | 2 | >128 | 2 | 64 | 8 | + | − | − | + | 122 | |||
| GEN 11R | 2 | 4 | 8 | 4 | 8 | − | + | + | + | 388 | 388 | ||
| GEN 12R | 2 | 16 | 8 | 32 | 8 | − | + | + | + | 388 | 388 | ||
| GEN 13Rc | 4 | >128 | 2 | 64 | 8 | + | − | − | + | 122 | |||
| GEN 14Rc | 4 | >128 | 2 | 64 | 8 | + | − | − | + | 122 | |||
| GEN 15R | 2 | 0.12 | 2 | 0.12 | 8 | − | − | − | − | ||||
| GEN 16R | 2 | 8 | 8 | 32 | 8 | − | + | + | + | 388 | 388 | 388 | |
| GEN 17R | 2 | 0.12 | 8 | 2 | 2 | − | − | + | + | 388 | 388 | ||
| GEN 1I | 0.25 | 0.12 | 16 | 16 | 0.5 | − | − | + | + | 295 | 295 | ||
| GEN 2I | 0.25 | 0.25 | 4 | 4 | 1 | − | − | + | + | 295 | 295 | ||
| GEN 3I | 1 | 0.12 | 2 | 0.06 | 4 | − | − | − | − | ||||
| GEN 4I | 1 | >128 | 16 | 16 | 4 | + | − | + | + | 388 | 388 | 388 | |
| GEN 5I | 1 | 128 | 16 | 16 | 4 | + | − | + | + | 388 | 388 | 388 | |
| GEN 6I | 1 | 128 | 16 | 16 | 4 | + | − | + | + | 388 | 388 | 388 | |
| GEN 7I | 1 | 128 | 16 | 16 | 4 | + | − | + | + | 388 | 388 | 388 | |
| GEN 8I | 0.12 | 0.25 | 4 | 0.12 | 1 | − | − | + | − | 388 | 388 | ||
| GEN 9I | 1 | >128 | 2 | 32 | 4 | + | − | − | + | 388 | 388 | ||
| GEN 12I | 0.12 | >128 | 16 | 32 | 0.5 | + | − | + | + | 388 | 388 | 388 | |
| GEN 13I | 0.25 | 0.12 | 2 | 0.06 | 2 | − | − | − | − | ||||
| GEN 14I | 1 | 0.12 | 2 | 1 | 4 | − | − | − | − | ||||
| GEN 15I | 1 | 0.12 | 2 | 0.06 | 4 | − | − | − | − | ||||
| GEN 16I | 0.12 | 1 | 2 | 0.5 | 0.25 | − | − | − | − | ||||
| GEN 17I | 1 | >128 | 16 | 16 | 4 | + | − | + | + | 388 | 388 | 388 | |
| GEN 18I | 1 | >128 | 16 | 16 | 4 | + | − | + | + | 388 | 388 | 388 | |
| GEN 19I | 0.12 | 32 | 2 | 16 | 0.25 | + | − | − | + | 332 | 332 | ||
| GEN 20I | 0.12 | 0.12 | 2 | 0.06 | 8 | − | − | − | − | ||||
| GEN 21I | 1 | 0.12 | 2 | 0.06 | 4 | − | − | − | + | ||||
| GEN 22I | 0.5 | 4 | 4 | 16 | 2 | − | + | + | + | 338 | 338 | 338 | |
| GEN 24I | 1 | 0.12 | 4 | 0.12 | 2 | − | − | − | + | 338 | |||
| GEN 25I | 1 | 0.12 | 2 | 0.06 | 2 | − | − | − | + | 338 | |||
a
PEN, penicillin; ERY, erythromycin; CHL, chloramphenicol; TET, tetracycline; CO-TX, co-trimoxazole.
b
Symbols: +, hybridization positive; −, hybridization negative.
c
Isolate belonging to Italian clone.