Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 19;14:5805. doi: 10.1038/s41467-023-41595-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Both heterozygous and homozygous Serine (S14A) knock-in (KI) mice were used in a and e whereas homozygous mice were used in b–d and f–h. a Heart weight normalized by tibia length at the indicated time points after TAC or sham. Sham: +/+ n = 5, KI/+ n = 7, KI/KI n = 5; 1 W: +/+ n = 5, KI/+ n = 6, KI/KI n = 5; 2 W: +/+ n = 6, KI/+ n = 8, KI/KI n = 5; 4 W: +/+ n = 5, KI/+ n = 7, KI/KI n = 5. b Wheat Germ Agglutinin (WGA) staining of the indicated heart tissues. Scale bar; 100 μm. c Quantitative analysis of relative cardiomyocyte size. n = 5. d Relative NPPA, NPPB, MYH7, and Rcan1.4 gene expressions. NPPA, NPPB, and MYH7: n = 6 (sham) and 9 (TAC). Rcan1.4: n = 4. e Calculated end-diastolic wall stress of the indicated mice 1-week post-TAC (n = 5). f Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of the indicated heart tissues. Arrows indicate TUNEL-positive nuclei. Scale bar; 100 μm. g Quantitative analysis of TUNEL-positive nuclei. n = 5. h Immunoblots showing cleaved caspase 3 and 9 expression levels in the heart after TAC or sham. Immunoblots were repeated at least three times using biologically independent replicates. In all graphs, WT is indicated by +/+ and knock-in (KI) is indicated by KI/+ (heterozygous) or KI/KI (homozygous). n represents biologically independent replicates. Two-way ANOVA with Tukey’s multiple comparison test. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns not significant. Adjusted p values for (a): 0.0015 (1 W, +/+ vs KI/ + ), 0.0001 (1 W, +/+ vs KI/KI), 0.0002 (2 W, +/+ vs KI/+). Adjusted p values for (c): 0.0012 (TAC 1 W, +/+ vs KI/KI), 0.0021 (KI/KI, sham vs TAC 2 W). Adjusted p values for (d): 0.0029 (NPPA: WT, Sham vs TAC), 0.002 (NPPA: TAC, +/+ vs KI/KI), 0.0251 (NPPB: TAC, +/+ vs KI/KI), 0.0004 (NPPB: KI/KI, Sham vs TAC), 0.0491 (MYH7: +/+, Sham vs TAC). Adjusted p values for (e): 0.0061 (WT vs KI/ + ), 0.0001 (WT vs KI/KI). Data are mean ± SEM. Source data are provided as a Source Data file.