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. 2023 Sep 19;14:5805. doi: 10.1038/s41467-023-41595-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Hemodynamic stress differentially alters intracellular Ca²⁺ dynamics in WT and KI cardiomyocytes, as shown in the Ca²⁺ transient amplitude, SR Ca²⁺ content, fractional Ca²⁺ release, and T₅₀. Cardiomyocytes were isolated from the indicated mouse hearts 1 day after TAC. WT baseline n = 21; WT TAC n = 18; KI baseline n = 24; KI TAC n = 20 from 3 hearts/group. Two-way ANOVA with Tukey’s multiple comparison test. b Representative traces of Ca²⁺ transient (F/F0) with 2-APB treatment in WT and KI cardiomyocytes 1 day after TAC. Orange dotted lines indicate the level of F/F0 at the time of 2-APB administration and arrows indicate the direction of change in F/F0. c Ca²⁺ transient amplitude before and after 2-APB treatment in cardiomyocytes 1 day after TAC. WT n = 13; KI n = 14 cells from 3 hearts/group. Two-sided paired t test (WT) or Wilcoxon matched-pairs signed rank test (KI). d Quantification of intracellular Ca²⁺ dynamics after 2-APB treatment in WT and KI cardiomyocytes 1 day after TAC. WT n = 13 and KI n = 14 cells (Ca²⁺ transient amplitude, two-sided Mann-Whitney U test); WT n = 9 and KI n = 10 cells (SR Ca²⁺ content and fractional Ca²⁺ release, two-sided unpaired t test); WT and KI n = 13 cells (T₅₀, two-sided Mann-Whitney U test) from 3 hearts/group. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns not significant. Adjusted p values for (a): 0.0216 (Ca²⁺ transient amplitude, WT, Baseline vs TAC), 0.0435 (Ca²⁺ transient amplitude, KI, Baseline vs TAC), 0.0071 (SR Ca²⁺ content, WT, Baseline vs TAC), 0.0007 (SR Ca²⁺ content, TAC, WT vs KI), 0.0423 (Fractional Ca²⁺ release, TAC, WT vs KI), 0.0001 (T₅₀, TAC, WT vs KI). Adjusted p values for (c): 0.0353 (KI, Pre-2APB vs Post-2APB). Adjusted p values for (d): 0.0373 (T₅₀, WT vs KI). Data are mean ± SEM. Source data are provided as a Source Data file.