Table 1. Proteins differentially abundant in Staphylococcus aureus periprosthetic joint infection (PJI) versus non-infectious arthroplasty failure (NIAF) sonicate fluid samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.
Proteins with increased and decreased abundance in S. aureus PJI compared to NIAF samples are unshaded and shaded, respectively. Statistical significance was determined using 2-sample t-test p-values ≤0.05 and log2fold-change values ≥2 or ≤−2. Data depicted are for NIAF (n=4) and S. aureus PJI (n=4) sonicate fluid samples.
| Protein description | Gene symbol | p-value | log2 Fold-change |
|---|---|---|---|
| Lactotransferrin | LTF | 0.0061 | 5.198 |
| Lipocalin-2 | LCN2 | 0.0032 | 5.001 |
| Myeloperoxidase | MPO | 0.0084 | 4.896 |
| Calprotectin (A9 subunit) | S100A9 | 0.0003 | 4.531 |
| Cathepsin G | CTSG | 0.0177 | 4.037 |
| Calprotectin (A8 subunit) | S100A8 | 0.0119 | 3.951 |
| Neutrophil elastase | ELANE | 0.0128 | 3.780 |
| Eosinophil cationic protein | RNASE3 | 0.0154 | 3.629 |
| Endoplasmic reticulum to nucleus signaling 1 | ERN1 | 0.0062 | 3.169 |
| Matrix metalloproteinase-9 | MMP9 | 0.0151 | 2.933 |
| Lysozyme C | LYZ | 0.0049 | 2.699 |
| Haptoglobin | HP | 0.0169 | 2.598 |
| Lamin-B1 | LMNB1 | 0.0007 | 2.492 |
| Glycogen phosphorylase, liver form | PYGL | 0.0059 | 2.471 |
| Leucine-rich alpha-2-glycoprotein | LRG1 | 0.0067 | 2.169 |
| Cathepsin D | CTSD | 0.0385 | −2.007 |
| Fatty acid-binding protein, heart | FABP3 | 0.0197 | −2.031 |
| Fructose-1,6-bisphosphatase 1 | FBP1 | 0.0218 | −2.039 |
| Fatty acid-binding protein, epidermal | FABP5 | 0.0222 | −2.063 |
| Collagen alpha-2(I) chain | COL1A2 | 0.0035 | −2.085 |
| Early endosome antigen 1 | EEA1 | 0.0360 | −2.111 |
| Heat shock protein beta-1 | HSPB1 | 0.0118 | −2.127 |
| Dihydrolipoamide S-succinyltransferase | DLST | 0.0256 | −2.304 |
| Branched-chain-amino-acid aminotransferase | BCAT1 | 0.0151 | −2.315 |
| Annexin A2 | ANXA2 | 0.0015 | −2.363 |
| Serpin B6 | SERPINB6 | 0.0439 | −2.384 |
| CD44 antigen | CD44 | 0.0020 | −2.451 |
| Dermcidin | DCD | 0.0435 | −2.458 |
| Ribonuclease pancreatic | RNASE1 | 0.0184 | −2.541 |
| Proteoglycan 4 | PRG4 | 0.0037 | −2.563 |
| Beta-hexosaminidase subunit beta | HEXB | 0.0494 | −2.662 |
| Osteopontin | SPP1 | 0.0099 | −2.690 |
| IFI30 lysosomal thiol reductase | IFI30 | 0.0237 | −2.798 |
| Cell surface glycoprotein MUC18 | MCAM | 0.0221 | −4.240 |
| Cartilage acidic protein 1 | CRTAC1 | 0.0025 | −5.297 |