Fig. 1.

Diffusion-corrected integral sedimentation coefficient distributions of A 1, 8, 12, 33 and 40 μM NET-1 in 50 mM Tris pH 7.5, 200 mM NaCl, as indicated by the color gradient from light to dark blue; B 7, 10, 19, and 22 μM NET-1 in 50 mM MES pH 6.5, 154 mM NaCl, as indicated by the color gradient from light to dark blue; C UNC-5 in Tris pH 7.5 (green) and MES pH 6.5 (red); D Molar extinction spectrum of NET-1 (blue) and UNC-5 labeled with Alexa Fluor-488 (red); E Crystal structure of NET-1 dimer structure (PDB code: 4OVE)