Table 1.

Discrete model genetic algorithm fitting results of NET-1 monomer–dimer equilibrium (Eq. 1)

Parameter		S (*10−13 s)	D (*10−7 cm/s)	f/f 0	ν(cm3/g)	Kd (μM)	RMSD
12.1 μm	Monomer	3.52 ± 0.04	5.90 ± 0.22	1.51 ± 0.06	0.71 ± 0.01	17.6 ± 3.6	0.00276
Tris pH 7.5	Dimer	4.83 ± 0.17	4.04 ± 0.09	1.75 ± 0.04
17.1 μM	Monomer	3.56 ± 0.03	5.09 ± 0.14	1.79 ± 0.06	0.66 ± 0.01	137 ± 26.1	0.00407
MES pH 6.5	Dimer	5.13 ± 0.10	3.66 ± 0.09	1.97 ± 0.05

All parameters were floated, and 95% confidence intervals were determined by Monte Carlo analysis with 100 iterations