Table 1.
Summary of the quantifications, number of cells, and experiments.
| Fig 1B | ||||
| Cell line | Condition | Rate of severe chromatin bridges (mean ± SD) (%) | Rate of chromatin bridges (mean ± SD) (%) | No. of cells (No. of experiments) |
| RPE1 | AdV-H2B | 0.00 ± 0.00 | 3.73 ± 6.41 | 34 (3) |
| 20 nM SiR-DNA 2 h | 16.04 ± 9.90 | 4.79 ± 4.18 | 42 (3) | |
| 20 nM SiR-DNA ON | 10.85 ± 10.72 | 6.08 ± 5.63 | 46 (3) | |
| 100 nM SiR-DNA 30 min | 36.31 ± 28.93 | 3.84 ± 3.36 | 52 (3) | |
| 100 nM SiR-DNA 2 h | 58.89 ± 18.36 | 4.45 ± 3.85 | 50 (3) | |
| 100 nM SiR-DNA ON | 37.79 ± 13.78 | 4.25 ± 4.02 | 52 (3) | |
| 500 nM SiR-DNA 30 min | 57.94 ± 8.36 | 3.52 ± 3.06 | 52 (3) | |
| 500 nM SiR-DNA 2 h | 73.28 ± 13.57 | 6.08 ± 5.63 | 29 (3) | |
| 500 nM SiR-DNA ON | 61.01 ± 12.12 | 2.78 ± 4.81 | 49 (3) | |
| U2OS | AdV-H2B | 0.00 ± 0.00 | 22.81 ± 5.97 | 49 (3) |
| 20 nM SiR-DNA 2 h | 16.87 ± 2.76 | 21.29 ± 9.78 | 52 (3) | |
| 20 nM SiR-DNA ON | 7.51 ± 7.70 | 18.61 ± 7.09 | 78 (3) | |
| 100 nM SiR-DNA 30 min | 34.47 ± 8.34 | 27.02 ± 5.58 | 40 (3) | |
| 100 nM SiR-DNA 2 h | 45.38 ± 5.04 | 22.51 ± 7.98 | 61 (3) | |
| 100 nM SiR-DNA ON | 17.87 ± 1.08 | 32.31 ± 11.90 | 72 (3) | |
| 500 nM SiR-DNA 30 min | 87.88 ± 10.50 | 6.06 ± 5.25 | 26 (3) | |
| 500 nM SiR-DNA 2 h | 88.43 ± 11.14 | 4.17 ± 7.22 | 26 (3) | |
| 500 nM SiR-DNA ON | 53.16 ± 16.71 | 16.24 ± 16.16 | 61 (3) | |
| DLD1 | AdV-H2B | 0.00 ± 0.00 | 10.72 ± 4.10 | 48 (3) |
| 20 nM SiR-DNA 2 h | 21.28 ± 12.12 | 8.44 ± 9.16 | 40 (3) | |
| 20 nM SiR-DNA ON | 15.00 ± 6.01 | 5.55 ± 4.81 | 29 (3) | |
| 100 nM SiR-DNA 30 min | 20.00 ± 10.00 | 10.00 ± 10.00 | 30 (3) | |
| 100 nM SiR-DNA 2 h | 41.98 ± 10.63 | 23.88 ± 7.99 | 39 (3) | |
| 100 nM SiR-DNA ON | 16.99 ± 5.58 | 3.81 ± 3.31 | 53 (3) | |
| 500 nM SiR-DNA 30 min | 54.55 ± 7.88 | 19.95 ± 4.44 | 35 (3) | |
| 500 nM SiR-DNA 2 h | 80.08 ± 3.21 | 17.35 ± 2.38 | 35 (3) | |
| 500 nM SiR-DNA ON | 18.95 ± 2.78 | 15.53 ± 0.46 | 58 (3) | |
| HeLa | AdV-H2B | 0.00 ± 0.00 | 9.11 ± 1.99 | 34 (3) |
| 20 nM SiR-DNA 2 h | 15.11 ± 4.41 | 17.94 ± 1.80 | 81 (3) | |
| 20 nM SiR-DNA ON | 0.00 ± 0.00 | 5.97 ± 5.17 | 63 (3) | |
| 100 nM SiR-DNA 30 min | 67.10 ± 5.78 | 2.56 ± 4.44 | 45 (3) | |
| 100 nM SiR-DNA 2 h | 72.48 ± 3.29 | 7.26 ± 2.90 | 71 (3) | |
| 100 nM SiR-DNA ON | 31.39 ± 9.87 | 10.33 ± 5.51 | 119 (3) | |
| 500 nM SiR-DNA 30 min | 83.05 ± 5.99 | 0.00 ± 0.00 | 28 (3) | |
| 500 nM SiR-DNA 2 h | 77.03 ± 10.42 | 3.94 ± 3.43 | 47 (3) | |
| 500 nM SiR-DNA ON | 41.82 ± 23.09 | 7.16 ± 8.28 | 76 (3) | |
| Fig 2B | ||||
| Cell line | Condition | Rate of severe chromatin bridges (mean ± SD) (%) | Rate of chromatin bridges (mean ± SD) (%) | No. of cells (No. of experiments) |
| RPE1 H2B-GFP/RFP-α-Tub | CTRL + 640 nm | 0.00 ± 0.00 | 0.00 ± 0.00 | 46 (3) |
| SiR-DNA | 0.00 ± 0.00 | 1.52 ± 2.63 | 62 (3) | |
| SiR-DNA + 640 nm | 48.15 ± 3.21 | 0.00 ± 0.00 | 39 (3) | |
| SiR-DNA + 640 nm (5 min) | 12.34 ± 2.65 | 2.56 ± 4.44 | 68 (3) | |
| U2OS H2B-GFP/mCh-α-Tub | CTRL + 640 nm | 0.00 ± 0.00 | 25.00 ± 8.33 | 42 (3) |
| SiR-DNA | 0.00 ± 0.00 | 28.62 ± 9.34 | 46 (3) | |
| SiR-DNA + 640 nm | 66.07 ± 4.26 | 19.69 ± 4.82 | 39 (3) | |
| SiR-DNA + 640 nm (5 min) | 14.49 ± 2.09 | 23.63 ± 7.44 | 48 (3) | |
| Fig 3C and D | ||||
| Cell line | Condition | Pole-pole velocity (mean ± SD) (μm/min) | No. of cells (No. of experiments) | |
| RPE1 H2B-GFP/RFP-α-Tub | CTRL | 1.64 ± 0.41 | 45 (3) | |
| 100 nM SiR-DNA w/o SCB | 1.51 ± 0.19 | 19 (3) | ||
| 100 nM SiR-DNA with SCB | 0.44 ± 0.34 | 19 (3) | ||
| U2OS H2B-GFP/mCh-α-Tub | CTRL | 1.23 ± 0.29 | 27 (3) | |
| 100 nM SiR-DNA w/o SCB | 1.15 ± 0.25 | 25 (3) | ||
| 100 nM SiR-DNA with SCB | 0.23 ± 0.19 | 26 (3) | ||
| Fig 4B and E | ||||
| Cell line | Condition | Rate of SCB w/o CENP-A (mean ± SD) (%) | Rate of SCB with CENP-A (mean ± SD) (%) | No. of cells (no. of experiments) |
| U2OS CENP-A-GFP/mCh-α-Tub | 100 nM SiR-DNA 2 h | 74.92 ± 19.95 | 25.08 ± 19.95 | 79 (5) |
| Cell line | Condition | Number of γ-H2AX foci per nucleus (mean ± SD) | No. of cells (no. of experiments) | |
| U2OS H2B-GFP | CTRL | 0.7 ± 1.0 | 46 (3) | |
| 500 nM SIR-DNA w/o SCB | 1.5 ± 1.6 | 35 (3) | ||
| 500 nM SIR-DNA with SCB | 4.0 ± 3.5 | 36 (3) | ||
| Fig S1B and C | ||||
| Cell line | Condition | Signal-to-noise ratio (mean ± SD) | No. of cells (no. of experiments) | |
| RPE1 | 20 nM SiR-DNA 2 h | 1.15 ± 0.02 | 90 (3) | |
| 20 nM SiR-DNA ON | 1.16 ± 0.05 | 90 (3) | ||
| 100 nM SiR-DNA 30 min | 1.26 ± 0.28 | 90 (3) | ||
| 100 nM SiR-DNA 2 h | 1.68 ± 0.21 | 90 (3) | ||
| 100 nM SiR-DNA ON | 1.18 ± 0.06 | 90 (3) | ||
| 500 nM SiR-DNA 30 min | 1.60 ± 0.34 | 90 (3) | ||
| 500 nM SiR-DNA 2 h | 2.02 ± 0.29 | 90 (3) | ||
| 500 nM SiR-DNA ON | 1.64 ± 0.15 | 90 (3) | ||
| U2OS | 20 nM SiR-DNA 2 h | 1.12 ± 0.01 | 90 (3) | |
| 20 nM SiR-DNA ON | 1.09 ± 0.06 | 90 (3) | ||
| 100 nM SiR-DNA 30 min | 1.37 ± 0.10 | 90 (3) | ||
| 100 nM SiR-DNA 2 h | 1.56 ± 0.20 | 90 (3) | ||
| 100 nM SiR-DNA ON | 1.33 ± 0.14 | 90 (3) | ||
| 500 nM SiR-DNA 30 min | 1.71 ± 0.03 | 90 (3) | ||
| 500 nM SiR-DNA 2 h | 1.90 ± 0.08 | 90 (3) | ||
| 500 nM SiR-DNA ON | 1.34 ± 0.05 | 90 (3) | ||
| DLD1 | 20 nM SiR-DNA 2 h | 1.12 ± 0.07 | 90 (3) | |
| 20 nM SiR-DNA ON | 1.09 ± 0.03 | 90 (3) | ||
| 100 nM SiR-DNA 30 min | 1.12 ± 0.03 | 90 (3) | ||
| 100 nM SiR-DNA 2 h | 1.39 ± 0.08 | 90 (3) | ||
| 100 nM SiR-DNA ON | 1.11 ± 0.05 | 90 (3) | ||
| 500 nM SiR-DNA 30 min | 1.57 ± 0.14 | 90 (3) | ||
| 500 nM SiR-DNA 2 h | 1.64 ± 0.11 | 90 (3) | ||
| 500 nM SiR-DNA ON | 1.33 ± 0.05 | 90 (3) | ||
| HeLa | 20 nM SiR-DNA 2 h | 1.18 ± 0.06 | 90 (3) | |
| 20 nM SiR-DNA ON | 1.04 ± 0.02 | 90 (3) | ||
| 100 nM SiR-DNA 30 min | 1.67 ± 0.16 | 90 (3) | ||
| 100 nM SiR-DNA 2 h | 2.33 ± 0.34 | 90 (3) | ||
| 100 nM SiR-DNA ON | 1.21 ± 0.11 | 90 (3) | ||
| 500 nM SiR-DNA 30 min | 3.60 ± 0.39 | 90 (3) | ||
| 500 nM SiR-DNA 2 h | 3.88 ± 0.37 | 90 (3) | ||
| 500 nM SiR-DNA ON | 1.67 ± 0.10 | 90 (3) | ||
| Cell line | Condition | NEB to AO duration (mean ± SD) (min) | No. of cells (no. of experiments) | |
| RPE1 | AdV-H2B | 20 ± 2 | 26 (3) | |
| 20 nM SiR-DNA 2 h | 21 ± 4 | 23 (3) | ||
| 20 nM SiR-DNA ON | 23 ± 6 | 30 (3) | ||
| 100 nM SiR-DNA 30 min | 21 ± 6 | 35 (3) | ||
| 100 nM SiR-DNA 2 h | 24 ± 5 | 32 (3) | ||
| 100 nM SiR-DNA ON | 24 ± 8 | 44 (3) | ||
| 500 nM SiR-DNA 30 min | 24 ± 8 | 30 (3) | ||
| 500 nM SiR-DNA 2 h | 29 ± 10 | 13 (3) | ||
| 500 nM SiR-DNA ON | 26 ± 7 | 25 (3) | ||
| U2OS | AdV-H2B | 33 ± 6 | 21 (3) | |
| 20 nM SiR-DNA 2 h | 27 ± 8 | 22 (3) | ||
| 20 nM SiR-DNA ON | 33 ± 7 | 26 (3) | ||
| 100 nM SiR-DNA 30 min | 23 ± 6 | 21 (3) | ||
| 100 nM SiR-DNA 2 h | 26 ± 6 | 22 (3) | ||
| 100 nM SiR-DNA ON | 33 ± 13 | 21 (3) | ||
| 500 nM SiR-DNA 30 min | 36 ± 5 | 13 (3) | ||
| 500 nM SiR-DNA 2 h | 31 ± 9 | 14 (3) | ||
| 500 nM SiR-DNA ON | 32 ± 6 | 18 (3) | ||
| DLD1 | AdV-H2B | 28 ± 7 | 21 (3) | |
| 20 nM SiR-DNA 2 h | 29 ± 7 | 18 (3) | ||
| 20 nM SiR-DNA ON | 34 ± 9 | 19 (3) | ||
| 100 nM SiR-DNA 30 min | 31 ± 7 | 20 (3) | ||
| 100 nM SiR-DNA 2 h | 35 ± 8 | 21 (3) | ||
| 100 nM SiR-DNA ON | 33 ± 6 | 21 (3) | ||
| 500 nM SiR-DNA 30 min | 35 ± 7 | 22 (3) | ||
| 500 nM SiR-DNA 2 h | 33 ± 8 | 20 (3) | ||
| 500 nM SiR-DNA ON | 32 ± 7 | 23 (3) | ||
| HeLa | AdV-H2B | 35 ± 6 | 28 (3) | |
| 20 nM SiR-DNA 2 h | 28 ± 7 | 21 (3) | ||
| 20 nM SiR-DNA ON | 32 ± 7 | 18 (3) | ||
| 100 nM SiR-DNA 30 min | 35 ± 10 | 20 (3) | ||
| 100 nM SiR-DNA 2 h | 39 ± 14 | 30 (3) | ||
| 100 nM SiR-DNA ON | 35 ± 10 | 36 (3) | ||
| 500 nM SiR-DNA 30 min | 42 ± 9 | 21 (3) | ||
| 500 nM SiR-DNA 2 h | 43 ± 13 | 15 (3) | ||
| 500 nM SiR-DNA ON | 42 ± 11 | 15 (3) | ||