Table 1.

Implication of metagenomics in plants using five recent studies

Research title	Sample size	Gene Extraction method	Seq. method	No. of Reads	References
Analysis of the bacterial populations living on Chinese Cordyceps: structure and estimated functional analysis	5 different samples (QF, XF, ZF, NaF, NyF)	High-throughput sequencing method	Illumina MiSeq PE250	591,672	[21]
Microbial Community Composition and Predicted Functional Assessment in Reclaimed Soil with Different Vegetation Types (e.g., Xiaoyi Mine Waste Dump in Shanxi)	Not mentioned	E.Z.N.A. DNA/RNA Isolation kit & E.Z.N.A Soil DNA Kit	16sRNA gene Sequencing	-	[20]
Intact communities of microbes living in harsh, essential for astrobiology anaerobic environments: Taxonomic and functional analysis	(6) IM, PPF2, PF2, RR, SS1, Hypersaline environment	Arch344F and Arch915R primers	Illumina MiSeq paired-end sequencing	37,516 reads	[19]
The genetic and enzymatic capability of the microbial community participating in the decomposition of a multifaceted microbial compound is shown by cultivation-independent and cultivation-dependent metagenomes	Not specified	ORF prediction	Shotgun metagenomics sequencing	18,762,958 reads (average of 1,563,580 reads/sample)	[22]

NB: This Table shows summary of 4 studies from different microbial communities through the Metagenomic tools are utilized to analyse diverse microbial communities in various environments and investigate their genetic and functional characteristics. Study 1: “Analysis of the bacterial populations living on Chinese Cordyceps” had five different samples (QF, XF, ZF, NaF, NyF) analysed using high-throughput sequencing (Illumina MiSeq PE250), resulting in 591,672 reads. Study 2: “Microbial Community Composition and Predicted Functional Assessment in Reclaimed Soil with Different Vegetation Types” used gene extraction kits and 16sRNA gene sequencing, but sample size and read count are not specified. Study 3: “Intact communities of microbes living in harsh anaerobic environments” examined six samples using Illumina MiSeq paired-end sequencing, generating 37,516 reads with Arch344F and Arch915R primers. Study 4: “Genetic and enzymatic capability of microbial community in decomposing multifaceted microbial compound” employed ORF prediction and shotgun metagenomics sequencing, resulting in an average of 18,762,958 reads (1,563,580 reads per sample) with an unspecified sample size.