TABLE 2.

Oligonucleotide primers used for amplification and sequencing of 16S rDNA

Primer	Sequence (5′ to 3′)	Positiona
TPU1	AGA GTT TGA TCM TGG CTC AG	EC8–27
TPU2	CCA RAC TCC TAC GGG AGG CA	EC334–353
TPU3	CAG CMG CCG CGG TAA TWC	EC519–536
TPU4	GGA TTA GAT ACC CTG GTA GTC C	EC785–806
TPU5	AAA CTY AAA KGA ATT GAC GG	EC907–926
TPU6	GGG CKA CAC ACG TGC TAC AAT	EC1220–1240
RTU2	TGC CTC CCG TAG GAG TYT GG	EC334–353
RTU3	GWA TTA CCG CGG CKG CTG	EC519–536
RTU4	TAC CAG GGT ATC TAA TCC TGT T	EC781–802
RTU5	CCG TCA ATT CMT TTR AGT TT	EC907–926
RTU6	ATT GTA GCA CGT GTG TMG CCC	EC1220–1240
RTU8	AAG GAG GTG ATC CAK CCR CA	EC1525–1544

^aNumbering is based upon Escherichia coli sequence.