L929 medium (L929M), only for BMDMs

Reagent	Final concentration	Amount
DMEM	N/A	430 mL
Fetal bovine serum (FBS)	10% (v/v)	50 mL
Sodium Pyruvate (100 mM)	1 mM	5 mL
Penicillin-Streptomycin (10.000 U/mL)	1% (v/v)	5 mL
GlutaMAX Supplement (100X)	1X	5 mL
HEPES	1%	5 mL
Total	N/A	500 mL

Store it up to 2 months at 4°C, pre-warm in a water bath at 37°C prior to use.