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Cycloheximide + O-Propargyl-Puromycin (CHX+OPP) working solution, only for BMDMs
| Reagent | Dilution factor | Amount |
|---|---|---|
| OPP stock solution | 1:400 | 0.25 μL |
| Cycloheximide | 1:500 | 0.20 μL |
| Cell culture medium | - | 99.55 μL |
| Total | N/A | 100 μL |
Prepare the solution in advance to rapidly add it to each well. The solution can be stored at 18°C–24°C and needs to be used immediately.
