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| Reagent | Dilution factor | Amount |
|---|---|---|
| anti-CD11b - BUV395 (clone: M1/70) | 1:200 | 0.6 μL |
| anti-CD45 - BV711 (clone: 30-F11) | 1:200 | 0.6 μL |
| LIVE/DEAD Fixable Near-IR for 633 or 635 nm excitation | 1:1000 | 0.12 μL |
| TruStain FcX (anti-mouse CD16/32) Antibody (clone: 93) | 1:200 | 0.6 μL |
| FACS Buffer | - | 8.08 μL |
| Total | N/A | 10 μL |
Prepare the solution immediately before use, protect it from light and keep on ice.
