Reagent	Dilution factor	Amount
Cell-Based Assay 5 FAM-Azide Stock Solution	1:1000	0.1 μL
Copper Sulfate Solution	1:1000	0.1 μL
Ascorbic Acid Solution	1:100	1 μL
Cell-Based Assay Buffer (1X)	-	98.8 μL
Total	N/A	100 μL

Prepare the solution immediately before use, protected from light and store at 18°C–24°C.