REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies (for FACS analysis)
Anti-CD11b-BUV395 (clone: M1/70) – dilution 1:200	BD Biosciences	Cat#563553
Anti-CD45-BV711 (clone: 30-F11) – dilution 1:200	BioLegend	Cat#103147
LIVE/DEAD Fixable Near-IR for 633 or 635 nm excitation – dilution 1:1000	Thermo Fisher Scientific	Cat#L10119
TruStain FcX (anti-mouse CD16/32) antibody (clone: 93) – dilution 1:200	BioLegend	Cat#101320
Biological samples
Brain cells (including microglia)	In this protocol	N/A
Bone-marrow-derived macrophages (BMDMs)	In this protocol	N/A
Chemicals, peptides, and recombinant proteins
Cell culture water	Sigma-Aldrich	Cat#W3500-500ML
DPBS (with calcium, magnesium, glucose, pyruvate)	Thermo Fisher Scientific	Cat#14287080
DPBS (without calcium, magnesium)	Sigma-Aldrich	Cat#D8537
Fetal bovine serum (FBS)	PAN-Biotech	Cat#P30-193306
Dulbecco′s modified Eagle′s medium-high glucose (DMEM)	Sigma-Aldrich	Cat#D5671
Sodium pyruvate (100 mM)	Thermo Fisher Scientific	Cat#11360039
Penicillin-Streptomycin (10.000 U/mL)	Thermo Fisher Scientific	Cat#15140122
GlutaMAX supplement	Thermo Fisher Scientific	Cat#35050087
EDTA, 0.5 M sterile solution, pH 8.0	VWR	Cat#E177-100ML
HEPES 1 M sterile	Thermo Fisher Scientific	Cat#15630056
HBSS, without calcium, magnesium, phenol red	Thermo Fisher Scientific	Cat#14175053
Cell Dissociation Buffer, enzyme-free	Thermo Fisher Scientific	Cat#13151014
Trypsin (2.5%), without phenol red	Thermo Fisher Scientific	Cat#15090046
Paraformaldehyde (PFA)	Sigma-Aldrich	Cat#158127
4% PFA/PBS	In-house	N/A
70% Ethanol	Carl Roth	Cat#T913.3
Lipopolysaccharides from Escherichia coli O111:B4 (LPS)	Sigma-Aldrich	Cat#L3024-10MG
Critical commercial assays
Protein Synthesis Assay	Cayman Chemical	Cat#601100
Adult Brain Dissociation Kit, mouse and rat	Miltenyi Biotec	Cat#130-107-677
Experimental models: Cell lines
L929 cell line [lot: 17110076 (07/2018)]	Sigma-Aldrich	Cat#85011425-RNA-5UG
Experimental models: Organisms/strains
C57BL/6J maintained in individually ventilated cages (IVCs); age- and sex-matched, co-housed	In-house	N/A
Software and algorithms
FlowJo software (v10)	BD Biosciences	https://www.flowjo.com/solutions/flowjo
Prism (v9.5.1)	GraphPad by Dotmatics	https://www.graphpad.com/scientific-software/prism/
Other
150 mm × 15 mm not TC-treated sterile Petri dish	Corning	Cat#351058
75 cm2 cell culture flask	Greiner Bio-One	Cat#658175
96-well clear bottom black TC-treated sterile microplate	Corning	Cat#3603
96-well clear V-bottom sterile microplate	Greiner Bio-One	Cat#651180
Cell strainer, pore size 70 μm	Sarstedt	Cat#83.3945.070
5 mL syringes	Braun	Cat#4606051V
Needles-27G 1/2″	BD	Cat#300635
Cell scraper	Greiner Bio-One	Cat#541070
5 mL round bottom polystyrene tubes (FACS tubes)	Corning	Cat#352054
Nalgene Rapid-Flow sterile vacuum filter units (0.2 μm pore size)	Thermo Fisher Scientific	Cat#567-0020
50 mL tubes	Greiner Bio-One	Cat#227-261
15 mL tubes	Greiner Bio-One	Cat#188-271
FACSymphony A5 – flow cytometer	BD Biosciences	N/A
Cell culture incubator at 37°C/5% CO2	Memmert	N/A
Plate centrifuge	Thermo Fisher Scientific	N/A