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. 2023 Sep 20;12(9):580–589. doi: 10.1302/2046-3758.129.BJR-2023-0102.R1

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© 2023 Author(s) et al.

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Fig. 2 — Obesity negatively regulates bone formation. a) Cancellous bone formation showed a significant decrease in the mineral apposition rate (MAR) as determined using a calcein double-labelling assay (n = 4). Magnification: 1,124×. b) Osteoblasts were detected by haematoxylin and eosin (H&E) staining and quantified; number of osteoblasts (N.Ob/B.Pm) and percentage of bone surface occupied by osteoblasts (Ob.S/B.Pm) were significantly decreased (n = 4). Magnification: 100× (top), 400× (bottom). c) Osteoclasts were detected by tartrate acid phosphatase (TRAP) staining and quantified; no difference in number of osteoclasts (N.Oc/B.Pm) or the percentage of bone surface occupied by osteoclasts (Oc.S/B.Pm) was detected (n = 4). Magnification: 100× (top), 400× (bottom). Data are expressed as means and standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001 compared with CTL (independent-samples t-test). BFR/BS, bone formation rate; CTL, control; DIO, diet-induced obese.