Fig. 3.

Cytotoxicity of NK cells. (A) LDH assay; activated NK cells toward MKN45 cells at different E: T ratios. The results demonstrated the most cytotoxic effect in 10:1 E: T (****P < 0.0001). Data reported as SEM for four groups independently. (B) Cytokine release assay of NK cells on day 21 by collecting the condition media. In comparison to control, the cytotoxic cytokine release of NK cells was ≈45 pg/mL and ≈27 pg/mL for IFNγ (**P < 0.01) and TNF α, respectively (***P < 0.001). Statistical significance was determined using a t-test (*P < 0.05).