FIG. 4.
Comparison of freshly prepared and stored reagents. Multiplex RT-PCR (with all the primer sets) was performed with a 10-fold dilution series of A/Taiwan/1/86 (H1N1) starting at 10−1 with both freshly prepared amplification mixtures (lanes 1 to 7) and amplification mixtures frozen for 6 months at −20°C (lanes 8 to 14). Results for influenza virus controls (A/Thessaloniki/1/95 H3N2; B/Harbin/7/94 B) and RSV controls (RSV type A Long, RSV type B VS1039) are shown. Lane 15, water control; Lane M, molecular size marker. Numbers on the left are in base pairs.