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. 2023 Sep 19;21:640. doi: 10.1186/s12967-023-04279-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — OPN expression increases in the lung of bleomycin-treated mice. A–E Bleomycin (5 mg/kg body weight) or saline were administered daily in mice (n≧4), and parameters analyzed on day 7, 14, 21 and 28. A Representative H&E, Masson and IHC staining of mouse lung tissue. Scale bar, 100 μm. Each group was given 5 different visual fields and the positive areas were quantified by average optical density value (n = 3), ****P < 0.0001, ns denotes no significance compared with day 7 group; means + SD. B Immunoblots of OPN, collagen and α-SMA of mouse lung tissue. C QPCR analysis of OPN gene (spp1) expression in the lung of bleomycin-treated mice. 18S was used as the reference gene, *P < 0.05; means + SD. D Concentration of OPN in peripheral blood determined by ELISA, ****P < 0.0001; means + SD. E Concentration of OPN in bronchoalveolar lavage fluid (BALF) determined by ELISA, *P < 0.05, **P < 0.01, ***P < 0.001; means + SD