Figure 4. Establishing the Local Cysteine Oxidation (Cys-LOx) method to analyze basal mitochondrial cysteine oxidation states.
A) Scheme of Cys-LOx method. B) Percent oxidation state of mitochondrial cysteines identified with Mito-Cys-LOx. C) Percent oxidation state of cysteines quantified in an exemplary mitochondrial protein. Experiments were performed in triplicate in iBMDM cells. See also Data S4 and Figure S4.