Fig. 2. Spectroscopic characterisation of as-isolated RirA variants. (A) Normalised UV-visible spectra of reconstituted wild-type RirA (black line) and as-isolated variants N8D (red line) and N8C (blue line) RirA. (B) Circular dichroism (CD) spectra for reconstituted wild-type RirA, and as-isolated variants N8D and N8C RirA, as labelled. The spectrum of S. coelicolor NsrR4 is also shown for comparison. Samples were prepared in 25 mM Hepes, 50 mM NaCl, 750 mM KCl, pH 7.5. Spectra were recorded using a 1 cm pathlength anaerobic quartz cuvette.
