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. 2023 Aug 30;17(17):16432–16447. doi: 10.1021/acsnano.2c12857

Figure 5.

Figure 5

In vitro assessments of targeting efficiency, cellular uptake, and genome-editing efficiency. (A) Schematic image of the BBB model in vitro. (B) Immunofluorescence images detected unmod-EVs, Ang-EVs, TAT-EVs and Ang/TAT-EVs uptake into LN229 cells after passing through a bEnd.3 monolayer. Scale bar, 10 μm. (C) The cellular internalization of the EVs were detected by flow cytometry. (D) The penetrating efficacy of Ang/TAT-sgGSS-EVs was evaluated in PDOs. (E) Frequency of GSS indel mutation. (F) Sequencing results of GSS gene editing in PDOs. (G) Detection of living and dead cells in PDOs. (H) The ratio of oxidized to nonoxidized lipids was assessed by flow cytometry. (I) The expression level of lipid peroxidation products (MDA). (J) Liperfluo staining visualized lipid ROS in cells after treatment. (K) The mitochondrial membrane potential MMP (Δψm) after treatment.