Skip to main content
. 2020 May 12;45(1):77–92. doi: 10.1247/csf.20012

Fig. 1.

Fig. 1

MON2 associates with both DOPEY1 and DOPEY2. a and b, HEK293 cells stably expressing DOPEY1-3FLAG (a) or DOPEY2-3FLAG (b) were transiently transfected with 3HA-sOGT or 3HA-MON2. Cell lysates were incubated with anti-FLAG beads, and precipitates were immunoblotted with the indicated antibodies. 3HA-sOGT was used as a negative control. c and d, Representative confocal images of EGFP-tagged DOPEY1 (c) and DOPEY2 (d). HEK293 cells were transiently transfected with the indicated constructs. Endogenous Giantin was immunostained as a Golgi marker. The enlarged images show magnified views of the boxed areas. Scale bars, 10 μm.