Fig. 5.

MON2 is required for optimal TfnR recycling. a, Immunoblotting of endogenous TfnR in HEK293 (WT), VPS35-KO and MON2-KO cells (left). Tubulin was used as a loading control. Quantification of TfnR in indicated cells (right). Data represent means±SE (n=3, **, P<0.01). b, HEK293 (WT) or MON2-KO cells were incubated with CF488A-Tfn at 4°C for 1 h, and then chased at 37°C. Data represent means±SE, with normalization by the cell number in each field. *, P<0.05; **, P<0.01, compared with control cells. c, Endogenous TfnR and SNX3 were immunostained in HEK293 (WT) and MON2-KO cells. Scale bars, 10 μm. d, Quantification of co-localization of TfnR with SNX3 by PCC. Data represent means±SE, ***, P<0.001 (two-tailed Student’s t test).