Figure 4.

Effect of cholesterol depletion on cell adhesion and rolling velocity of lung cancer cell lines on E-selectin-coated surfaces under physiological conditions in a flow chamber. A: of attached cells decreased for all the NSCLC cells upon MβCD and Simva treatments, whereas the SCLC cell line remained unaffected. B: the rolling velocities of H1299 and H23 cell lines significantly increased only through MβCD treatment. Values are means ± SE for n = 4 independent experiments. Statistical analysis was performed by one-way ANOVA with Tukey’s HSD post hoc test. *Statistical significance compared with the relative control (P < 0.05). **Statistical difference compared with nontreated cells (P < 0.05). HSD, honest significant difference; MβCD, methyl-beta-cyclodextrin; NSCLC, non-small cell lung cancer; SCLC, small cell lung cancer.