Figure 6.

CD44 expression of lung cancer cells after mβCD, Simva, and SMase treatments. A: upon treatments with mβCD and Simva, CD44 was cleaved from the surface of almost all the NSCLC cells except for the SCLC cell line (with no CD44 expression; A). Histograms are representative of n = 3 independent experiments. B: mean fluorescence intensity (MFI) of CD44 expression showed a decrease for all NSCLC cell lines with mβCD and Simva treatments, whereas no change was observed for the SCLC cell line before and after treatments. Values are means ± SE for n = 3 independent experiments. Statistical analysis was performed by one-way ANOVA with post hoc Tukey’s HSD test comparison. *Statistical significance compared with nontreated control cells (P < 0.05). HSD, honest significant difference; MβCD, methyl-beta-cyclodextrin; NSCLC, non-small cell lung cancer; SCLC, small cell lung cancer.