Figure 4.

Enzymes related to the metabolism of histamine are expressed in the renal cortex of normal-salt (NS; 0.4% NaCl) or high-salt (HS; 4% NaCl) diet-fed male Dahl salt-sensitive rats. A–C, left: histamine decarboxylase (HDC; A), histamine N-methyltransferase (HNMT; B), and diamine oxidase (DAO; C) expression was confirmed with Western blot analysis using renal cortex samples of male Dahl salt-sensitive rats fed NS vs. HS diets. Each lane represents a sample from an individual rat. Pierce-stained membrane used for total protein analysis is shown below the membrane probed with the antibodies. A–C, right: densitometry analysis of the Western blots shown on the left; n = 10 individual animals per diet group. Boxes indicate means ± SE; whiskers indicate SDs. One-way ANOVA with a Holm-Sidak post hoc test was used for statistical analysis; a.u., arbitrary units.